Consider the DNA sequence evolution of the 6-phosphogluconate dehydrogenase, decarboxylating (gnd) gene in the following organisms:
Encephalitozoon cuniculi (Fungus)
Dictyostelium discoideum (Slime mold)
Synechococcus elongatus (Cyanobacteria)
Cronobacter dublinensis (Proteobacteria)
Candidatus Solibacter usitatus (Acidobacteria)
Leptotrichia goodfellowii (Fusobacteria)
Borrelia garinii (Spirochaetes)
Phycisphaera mikurensis (Planctomycetes)
Mycobacterium tuberculosis (Actinobacteria)
Anaerolinea thermophila (Chloroflexi)
Chlamydia trachomatis (Chlamydiae)
Lactococcus lactis (Firmicutes)
Before running any tests, think about whether you believe a molecular clock would be a valid assumption with this dataset. Then test whether a molecular clock could apply to these sequences. What is your conclusion? Is it surprising to you?