Create alignments of the 6-phosphogluconate dehydrogenase, decarboxylating (gnd) protein and DNA sequences for the following species:
Encephalitozoon cuniculi (Fungus)
Dictyostelium discoideum (Slime mold)
Synechococcus elongatus (Cyanobacteria)
Cronobacter dublinensis (Proteobacteria)
Candidatus Solibacter usitatus (Acidobacteria)
Leptotrichia goodfellowii (Fusobacteria)
Borrelia garinii (Spirochaetes)
Phycisphaera mikurensis (Planctomycetes)
Mycobacterium tuberculosis (Actinobacteria)
Anaerolinea thermophila (Chloroflexi)
Chlamydia trachomatis (Chlamydiae)
Lactococcus lactis (Firmicutes)
from the data you gathered in the previous homework assignment. Be careful: some of the DNA sequences that I gathered were actually reverse complements. Also, start and stop codons should be stripped before the analysis.Additionally, some start DNA codons were noticably incorrect but easy to fix and I still recommend throwing the start codon out. It's just usful to know what is suppose to be "atg" when something else is recoded at the start. Useful tools are available at:
Clustal Omega online from EMBL-EBI
and
DNA sequence manipulation